Nature Methods Article!

[GenocideAlive]

Well, if any of you wonder why I slave away at a bench all day, here's why: I'm in the business of siRNA validation, and we're pioneering a technique and business process for high-throughput (lots of samples) validation. It's relatively fresh territory and very lucrative for companies, so there's a lot of scrutiny right now.

Journal: Nature Methods, 2006. Is. #385.

High throughput screening of RNAi screening by time-lapse imaging of live human cells.

While I'd love to link the article and all that, Nature requires a subscription to access online articles and I'm not entirely sure what constitutes their propriety rights. I'm pretty sure I'll get myself and Warboards in some trouble if I copy-paste.

[hammocksleeper]

fair use guidelines say you can post an excerpt of a couple paragraphs or even a whole page, depending on how long the article is.

[GenocideAlive]

I have no idea what "fair use guidelines are", but I'm inclined to think that the subscription permits myself usage, but putting it on a forum for the public could be considered a publishment. If anybody could offer me reasonable assurance, I'd be more inclined to comply with the post.

[LinkTheGameFreak]

I have no idea what "fair use guidelines are", but I'm inclined to think that the subscription permits myself usage, but putting it on a forum for the public could be considered a publishment. If anybody could offer me reasonable assurance, I'd be more inclined to comply with the post.

Would you really get in trouble if you posted it here? I say post it cuz the chances are that no one will really care if you put some excerpts here - fair use, schmare use... that kind of crap ruins alot of good things :mad:

[hammocksleeper]

btw it's vol. 3, issue 5, page 385 that you want to refer people to. my university has an online subscription to the journal, so here's a summary for y'alls enlightenment. i have no fucking clue what any of it means.

RNA interference (RNAi) is a powerful method to
determine the function of specific genes and proteins
by triggering degradation of target mRNAs. But the
methods of measuring the effect of gene knockdown
by RNAi now rely on end-point assays. Therefore,
these assays may miss important or complex transient
phenotypes. Ellenberg and colleagues describe
a combination of on-chip short interfering RNA
transfection and live-cell fluorescent imaging for
high-throughput screening of large numbers of gene
knockdown phenotypes. Visualizing the effect of RNAi
in real-time using live cells offers a valuable addition
to what is currently achievable with end-point assays.

[GenocideAlive]

If I get into trouble, it's hammock's fault.

...I'm 74kb over the limit. >_< !(#@$*&)!#@(*$